Initially, cells are dissociated from liver tissues into single-cell suspensions using in vivo enzymatic perfusion or ex vivo digestion methods. The cells of interest are then enriched from the ...

Spatial transcriptomics (ST) technologies reveal the spatial organization of gene expression in tissues, providing critical insights into development, neurobiology, and cancer. However, the high cost ...

Spatial transcriptomics is a technique that provides information about gene expression patterns within intact tissues. This technology employs various methodologies, including in situ sequencing (ISS) ...

Rare cancers encompass a heterogeneous group of malignancies characterized by low incidence and prevalence but, often, disproportionately high mortality ...

Fei Chen and Chenlei Hu at the Broad Institute of MIT and Harvard have developed a new imaging-free spatial transcriptomics technology that tracks the diffusion of DNA barcodes between beads in an ...

Single-cell RNA transcriptomics allows researchers to broadly profile the gene expression of individual cells in a particular tissue. This technique has allowed researchers to identify new subsets of ...

Knowing the location of a gene within intact tissue or a single cell allows scientists to unlock unknown cellular functions. This information is often lost in most genetic sequencing techniques, but ...

The liver is remarkable for its ability to regenerate after injury, yet when this process fails, acute liver failure (ALF) carries devastating outcomes. Traditional research methods, reliant on bulk ...

Andreas Pfenning discusses the techniques being developed and used to study neuronal heterogeneity and the therapeutic potential of his work.

A single-cell atlas of brain aging epigenetics has mapped methylation, chromatin, and gene activity changes across 36 cell ...