In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

Escherichia coli (E. coli) has been commonly used for the production of biopharmaceuticals. Among the impurities that must be monitored in biopharmaceuticals is residual host-cell DNA (HCD). This ...

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

Real-time PCR has many advantages compared with antigenemia and qualitative PCR assays for detecting cytomegalovirus (CMV) infection in patients following SCT. However, the procedure used in each ...

The polymerase chain reaction (PCR), invented about three decades ago, soon entered mainstream use thanks to an ongoing series of refinements. One particularly important refinement, introduced about ...

Real-time PCR is an important tool for life scientists looking to evaluate gene expression. While the chemical reaction underpinning the technique is relatively simple, real-time PCR instruments can ...

The firm said it is the only analysis method capable of providing and analyzing multi-Ct (threshold cycle) values in a single detection channel, which offers simultaneous amplification of multiple ...

All three patients had clinical and biological features highly suggestive for CML. Bone marrow examination of the third patient also revealed 30% blasts with myeloid antigens. Cytogenetic analyses ...